Composite

Part:BBa_K4760003:Design

Designed by: Xianghai Jiang   Group: iGEM23_SJTU-BioX-Shanghai   (2023-10-09)


ClyA-PbrR-GGGGS-His10


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ClyA is an endotoxin and may appear hazardous in certain cases. Yet considering ClyA-containing OMVs are widely utilized in publications on OMV engineering for medical usage, we decided that this possible hazard is minor enough to ignore.


Source

The ClyA sequence is from E.coli K12 genome; the PbrR sequence is originated from Cupriavidus metallidurans CH34 strain. Both are synthesized and optimized to most preferred codon usage of E.coli BL21 strain.

References